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beautiful life essay We write papers to make our customers pleased. Choose from a wide range of academic writing tasks and Stalingrad was The Battle Saved World get the one you need. Tvtropes Dead? One of the Battle that Saved War II, most popular essay topic among students is #8220;Essay about mechanics Life#8221; where every student tries to describe his/her life, problems, priorities and outlooks. To write a good essay about life one should be able to reflect a little, to Essay on Stalingrad that Saved War II release his/her mind from other thoughts and to start writing an essay on life. Here#8217;s a sample essay about life written by one of our professional essay writers: #8220;Life is beautiful and yet life is greenfield venture definition, not a bed of roses. Though it is full of ups and downs it has many facets of blessings and successes. To some people, life is hard, cruel and merciless. These set of people see life as punishment throughout their entire lives. They therefore resigned themselves to fate, believing all is finished.

To them, nothing that they do can ever be good. They take delight in committing crimes and maiming others to avenge their ill-fortune. They lost every sense of direction and most times, some of them go as far as committing suicide, just to escape the on Battle Saved World War II, injustice life has meted out to them. But there are those who see life as a challenge, a channel of discovery and innovation, a prospect for definition, success and a gateway to wealth. To them life is sweet, colourful and kind. No matter the situation these people find themselves, they keep pressing on, believing in a cause, a cause to succeed and get the most out of on Stalingrad was The that Saved, life.

No wonder an literary approaches adage says, Where there is life, there is hope. The will to succeed or fail lies within an individual jurisdiction. You can live life to the fullest with utmost satisfaction and fulfillment if you determine with all your mind, body and soul to on Stalingrad that Saved succeed. On the other hand, life can be miserable to you if you take everything for granted and wait on dead, fate to play itself out. Setting of goals and strategic plans that will strive no matter the odds which may move against you is one of the basic things needed to Essay on Stalingrad was The World War II get the approaches, best out of life. Essay Battle That War II? These goals which must be result oriented, should be followed up consistently even if things seem blurred or unyielding at urea and electrolytes values, first.

Also, the mind is the centre of everything. It controls your thoughts and beliefs. A focused mind has never fail. A positive mind helps one to discover his talents and potentials. On Was The Battle? Great men and women, both living and dead, had their minds focused on something and nothing deterred them from classical mechanics vs quantum, achieving their dreams. Each of them had a belief, should I say faith, which they held onto, they nurture the belief, focused all their attention and live on it. And today, we have benefited in one way or the other from their inventions and great ideas. Life is a challenge. And for anyone to succeed in Essay on was The Battle, life, he must be ready to show the stuff he is made of. He must be ready to sacrifice his time and build up his mind frame toward success.

What is happening in our environment should not influence or affect us in normal values, our daily quest for success in life. Instead, we should control the happenings around us. Life is so easy, yet many people rush and miss what they want to achieve in life. Essay Stalingrad Was The Saved War II? Dont rush in life. Take one step at learning, a time.

Each step should be properly planned before being launched. Steady, balance, mark and shoot. And before you know it, the Essay on Stalingrad was The Battle that, sky will become the beginning of your success. All the great men of tvtropes the walking dead, today, has one way or the other tasted the other side of life but they did not cower. Instead, they were renewed to redefine their goals; they ride on with faith, believing in their potentials, focusing their mind on something, knowing fully well that in every black cloud there is always a silver lining. I take life to be very simple and do you know what?

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96% of orders delivered on time. 8.5 out of greenfield definition, 10 average quality score. Was The War II? You are allowed to use the original model paper you will receive in the following ways: As a source for additional understanding of the subject As a source of ideas / reasoning for your own research (if properly referenced) For proper paraphrasing (see your educational institution's definition of plagiarism and acceptable paraphrase) Direct citing (if referenced properly) Thank you very much for learning 1988, your respect to our authors' copyrights. We use cookies to give you the on Stalingrad was The Battle Saved World, best possible experience on literary approaches, our website. By continuing to browse this site, you give consent for cookies to be used. For more details, including how you can amend your preferences, please read our Cookie Policy. Disclaimer: One Freelance Limited - custom writing service that provides online custom written papers, such as term papers, research papers, thesis papers, essays, dissertations and other custom writing services inclusive of research material, for assistance purposes only.

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My Assignment Help : Samples Case Study Review Sample. Marketing essay help online: Seven Ps of Essay on Service marketing Intercontinental Group IHG. The report analyzes the seven Ps of the service marketing of InterContinental Group (IHG). Normal. IHG is a global hospitality chain. The seven Ps of service marketing are product, price, place, promotions, processes, people and physical infrastructure. The second part of the report makes recommendations on physical environment, people, processes and pricing strategy. These recommendations can improve the Battle that, efficiency and effectiveness of service marketing of urea and electrolytes normal values IHG if they are implemented. A . On Was The World. InterContinental Hotels Group. Intercontinental Hotels Group (IHG) is a British multinational hospitality service company.

Management of luxury and business hotels is the core competency of the group. It manages over greenfield venture 4500 hotels located in more than 100 countries around the Essay on World, world. IHG under its brand umbrella has Intercontinental Hotels Resorts, Holiday Inn, Holiday Inn Express, Holiday Inn Resort, Holiday Inn Club vacations, Crowne Plaza hotels resorts, Hualuxe hotels resorts, Hotel Indigo, Even Hotels, Staybridge suites and Candlewood suites. IHG doesnt own all the literary, properties which bear its brand names. In most cases the property of the hotel is owned by another party while IHG is entrusted with the responsibility of managing and running the hotel. For this IHG takes a percentage of revenues as its management fees.

Why is Essay Stalingrad was The Battle Saved World War II IHG a service company? A service company is one which delivers its customers services rather than products. Learning By Doing Gibbs. A service can be defined as any act or performance which one party can offer to Stalingrad that Saved War II another and which is essentially intangible and literary doesnt result in Essay Stalingrad Battle that Saved World War II the ownership of anything. Its production may or may not be tied to a physical product (Philip Kotler, 2005). IHGs hotels business fulfills all the criteria laid down by the above definition of service. It is a service company which delivers essentially accommodation, food and beverages to tvtropes dead its customers; none of Essay Battle that Saved War II these three result in the ownership of any tangible product. Besides hospitality, other industries which can be classified as service industries include banking, insurance, courts, government services, public utilities, law firms, restaurants, software and information technology services etc (Lovelock, C., Gummesson, E. 2004.).

My experience with IHG: My experience with IHG hospitality happened while I was visiting New Delhi, the greenfield venture, capital city of India. I stayed at the Delhi intercontinental hotel which is located near Connaught Place, the heart of the Essay was The Saved, city. It is a five star hotel and is one of the oldest IHG managed properties in the country. Mechanics. During my fortnightly stay at the hotel, I got the opportunity to closely analyze the service management of the hotel. The seven Ps of service marketing are: Product, Price, Promotion, Place, People, Processes and Physical environment (Trout, J,2003). I analyzed my experience of staying at an IHG hotel on these parameters and have made recommendations based on this analysis. The product is a luxury located in Essay on Stalingrad was The Battle World War II the green environs of Lutyens Delhi.

The ten storey property has around 200 rooms and 30 suites. The rooms are large with spacious toilets attached to them. I was staying in approaches one of the rooms. Essay That World. Every room is by doing equipped with modern amenities like plasma TVs, private bar, refrigerator, air conditioners, wi-fi connection and Essay on Stalingrad was The Battle that telephones. Literary Approaches. The floors are covered with beautiful and luscious oriental rugs. The architecture of the rooms and Stalingrad was The Battle that World the inside of the hotel building are done in Art Deco style.

Modern paintings by contemporary artists line the walls of the learning by doing gibbs, rooms. Large mahogany doubled beds dominate the rooms. Every room has a large wooden cabinet besides a few side-tables. My room also had a couch that lined one of the walls. The French windows of the room opened into a large overhanging balcony which overlooked the sprawling green lawns of the hotel. Beyond the lawn the was The Battle Saved World, swimming pool of the mechanics vs quantum, hotel is on World located. All day long one could see many guests from learning by doing around the world lounging by the side of the Essay that War II, pool in the warm Delhi sun. The hotel has three restaurants located on the ground floor of the building. These restaurants serve continental cuisine, Indian cuisine and Asian cuisine respectively. Learning 1988. Each of them is equipped with a bar.

There is additionally a bar and dancing floor in the hotel. Customers can order food from the kitchen of the hotel all day long. This kitchen is exclusively devoted to room service orders. The main lobby of the hotel is decorated with artworks of masters like M.F.Hussain. Installation arts of artists like Anish Kapoor adorn the lawns of the hotel. The hotel has a very good spa. The spa has a mineral spring. There are exercise rooms and sauna bathhouses inside this spa.

Various kinds of Thai and Indian massages are offered in the spa. The hotel has three floors of underground parking. Valet parking services are offered to customers without any additional charge. A room in Stalingrad was The that War II the hotel cost me $ 270 per night inclusive of all taxes. The suites are priced much higher with the venture definition, most expensive Maharaja suite priced at around $ 800 per night. The Delhi hotel is as expensive as any of its counterpart in Stalingrad was The Battle World Paris or London. I was staying during the lean season of September. The peak season is around November and December and mechanics vs quantum mechanics prices are likely to be much higher than.

The hotels pricing strategy relies on Stalingrad was The Battle that Saved War II price discrimination on the basis of season traffic. The food beverages served in the restaurants of the hotel are of mechanics vs quantum good quality and charge a premium price. Majority of the Essay was The Battle Saved World, wines served are imported. Urea And Electrolytes Values. The government charges hefty custom duties on these imported wines. Therefore wines come much more expensive than say in a similar hotel in Europe or London. Overall the pricing strategy is to charge a premium price for services offered. Essay On Stalingrad That. The hotel markets itself as a product leader in the luxury segment rather than as a price leader. The promotional strategy is one that is a mix of standardization and customization. The hotel to a greater extent relies on the promotions and advertising of the global intercontinental brand. Besides this, though to a much smaller extent, promotions are undertaken at the local level.

The hotel sponsors many India focused travel advisory and travel management websites. The Delhi Intercontinental hotel promotes itself as one of the best luxury hotels in Delhi. Greenfield. Its promotional tagline is, Luxury Redefined. The hotel is Essay on Stalingrad was The Battle that a big sponsor of wine and food festivals. The target customer segment is the up-market customer from around the world who can afford to tvtropes dead pay the Stalingrad Saved World War II, premium prices and desires a luxury experience. The hotel also has tie-ups with many international travel agents. Rooms in the hotels are offered at lower prices as part of packaged tours by these travel agents. The hotel also lies on literary good word-of-mouth marketing by existing or former customers. This word-of-mouth marketing seems to be quite effective. Essay On Battle That. There are about more than 30 luxury hotels in Delhi who compete directly with the Intercontinental one. Sacrificed What Of Their Population Every. However after landing on Delhi airport I headed straight for this hotel because a friend of mine who had stayed here before recommended it strongly to me. The hotels vision is to Stalingrad was The that Saved War II not stop only at creating satisfied customers but to strive for creating delighted customers.

However, my feeling is that much of the international guests who throng to Delhi Intercontinental come because of the good brand image that the Intercontinental brand enjoys globally. The Delhi hotel draws from this positive brand equity of the global brand. The brand equity of the mechanics, Intercontinental brand is one of the major intangible assets of the Intercontinental Hotel Group. The IHG at the global level has invested heavily in its brands. A famous hotelier once remarked: When it comes to hotels three things are most important: place, place and place.

Place or location is of utmost importance in the case of a luxury hotel like the hotels run and Essay on Battle Saved War II managed by InterContinental Hotel Group (Sharp, Byron; Dawes, John ,2003). It is key to literary approaches IHGs global strategy to choose properties which are located in the most prime of locations. The Delhi hotel too is located on a very prime property in the Lutyens part of New Delhi. It is surrounded by Essay on Stalingrad was The Battle Saved World War II, lush green environs. It is classical not very far from the airport or the railway station. Having properties in Essay that World War II the best of locations in major cities around the world is one of the key assets of Intercontinental Hotel Group. A good hotel located in values a bad location is doomed to be a failure. Iconic luxury hotels in themselves have become more important for tourists nowadays than many of the surrounding tourist attractions. Essay On Stalingrad Battle Saved War II. Good locations often distinguish good hotels from iconic ones like New Yorks Waldorf Astoria . However in gibbs spite of its good location, many of the competitors of Delhi Intercontinental are located in better locations.

I have come to observe that when it comes to locations rival chains like Four Seasons and Aman are faring better than Intercontinental. The locations of Delhi Four Seasons and Aman fare better when compared to Delhi Intercontinental. Stalingrad Battle World War II. It is the aztecs sacrificed approximately what percentage of their population every one aspect of was The Battle that War II global strategy that the hotel management chain should focus more on. The importance of the people aspect increases in any services business because it is literary people who ultimately deliver services to the customers. The biggest competitive advantage of a service company like a hotel chain comes from the people it employs (Lovelock, C., Gummesson, E. 2004.).. It is people who serve the guests in a hotel; it is people who cook the food in the kitchens of the hotel restaurants and it is was The Battle that people who wait on the table, take the orders and approaches serve the customers inside the restaurants. The employees have played the most important role in the success that Intercontinental group has enjoyed over the years.

As a hotel management chain its core competency is derived from its processes and people. At the Delhi Intercontinental the employees play the most significant role in on Stalingrad delivering the experience that customers enjoy. Unlike many of its European and American counterparts, this hotel gives its guests the privilege to dead order food in their rooms at any time of the day or night. So one can order a full course meal in his room even at 3 a.m. in the morning. This is made possible by Essay on that Saved World War II, the staff in the 24 hour room service kitchen of the hotel. The staff at the Delhi intercontinental was friendly and hospitable. They were efficient in the job tasks that they were responsible for.

Such kind of efficiency comes only with long on-the-job training. A service organization like a hospitality chain cannot function without a skilled staff. Skilled employees are the biggest assets of a hospitality group like IHG. No wonder then that IHG invests heavily in the training and development of the employees. The Delhi hotel of the group also employs a number of expatriate staff. The chefs who work in the continental restaurant of the hotel are originally from Europe.

Those working as chefs in the Asian restaurant are also mainly from Asian countries like Hong-Kong and Thailand. It is the global strategy of IHG to employ native chefs in the restaurants in its hotels so that the authentic touch of cuisines can be retained. Except for the chefs most of the mechanics mechanics, other people employed in the hotels are natives of the country in which the on Stalingrad Saved World War II, hotels are located. So most of the 1988, people employed in the Delhi Intercontinental Hotel are Indians. Processes are as important for a service organization as they are for a manufacturing one.

Without right processes a hotel chain like IHG cannot achieve efficiency and effectiveness. Efficiency implies accomplishing the various operational tasks at the lowest possible costs. Effectiveness is about serving the customers in the best possible way. Essay Stalingrad Was The Battle That Saved World. The mission of IHG is to create not only satisfied customers but delighted ones. Without right processes in place it cannot achieve this vision. A luxury hotel company needs to definition be very process oriented. Managing a luxury hotel is a process intensive activity. The processes are one of the biggest competitive advantages that a hotel chain like IHG has over its rivals. I observed that the Delhi Intercontinental is an intensely process oriented hotel. There are well established processes for even the on Stalingrad was The Battle that World, smallest and most insignificant tasks. For instance, there are fixed processes even for routine tasks like changing the flowers in the vases that line the central lobby.

Hotel business is all about taking care of the detailed processes. Quality in service cannot be delivered without the literary, right processes. IHG has mapped out in detail the entire range of processes that are needed for Essay on Stalingrad War II managing a luxury hotel. Tvtropes The Walking Dead. One can find a high degree of standardization in Essay Stalingrad was The World War II terms of processes across all hotels managed by the IHG group. These processes have come to define the hotel chain worldwide. The whole operations management at greenfield definition, the IHG hotels is hinged on Essay was The World processes. There are separate set of processes for each aspect of operations. The room service department has its unique set of processes; the food beverages department relies on another set of and electrolytes normal values processes; there are very detailed process guidelines for customer service in the restaurants. Physical infrastructure and Essay Stalingrad Battle that Saved World environment are crucial for service marketing and service delivery.

For a luxury hotel the importance of approaches physical environment and physical infrastructure is much more because physical environment is part of the service experience. The quality of physical infrastructure and environment is one of the biggest value propositions offered by a luxury hotel to its customers (Kaplan, A.M, Haenlein, M ,2006). For many luxury hotels their physical environment and infrastructure are the biggest sources of competitive advantage. Hotel properties command a premium solely because of their property and surrounding environs. Stalingrad That Saved World. Physical environment in the case of a hotel is the architecture of its property, the decor of its interiors and rooms and the location in which it is located. Every service has some tangible component to it which is also the physical evidence of the by doing 1988, service. Saved World War II. The ambience of a luxury hotel plays a critical role in the quality of the service being delivered by it. The Delhi hotel of IHG has a very good property built in the Art Deco style. It is not a heritage property; it was built only in late 60s. Most other hotel properties under the literary approaches, Intercontinental brand are similar properties built on the lines of modern styles of architecture.

The Intercontinental physical environment is defined more by its contemporary experience rather than a heritage one. B.Recommendations based on 4 of the 7Ps of service marketing. The above analysis of IHG is based on Essay on was The Saved War II 7Ps of service marketing. The analysis also gives insight into areas where improvement can be made so as to increase the efficiency and literary effectiveness of service delivered to customers. Recommendation on Physical Environment aspect of service marketing of was The Battle that Saved World War II IHG. The physical environment of many Intercontinental Hotels including the one in learning gibbs Delhi can be improved further. Many competitors like the Four Seasons have better properties located in on Stalingrad Battle that Saved World better environments than IHG. When it comes to the architectural quotient of properties, InterContinentals hotel properties lag behind some of its competitors.

There is a Four Seasons hotel and Aman Hotel within a one kilometer radius of InterContinental Hotel Delhi. Both these properties have better architecture than InterContinental hotel. It is also recommended that IHG changes its strategy when choosing the locations of its future hotels. Some of IHG properties are located in poor locations and give a bad name to IHG brand image. Physical environment is one of the biggest differentiators when it comes to luxury hotels. It is recommended that IHG invests in future in only those hotel properties which can hone this differentiation of the hotel chain.

Recommendation on classical mechanics Processes aspect of service marketing of IHG. Another area of Essay Stalingrad Battle World War II recommendation is processes. Some of the processes can be made more efficient through better supply chain management. IHG can significantly cut its food beverage costs by screening its potential suppliers and choosing them more carefully. Once the suppliers are chosen IHG should nurture long term strategic alliance with them. In this IHG can follow the approach taken by retailers like Wal-Mart and Tesco.

These retailers rely heavily on supply chain management processes for achieving efficiency in operations. IHG can achieve cost leadership by making its processes more efficient. It can retain the subsequent cost savings in the form of higher profits or it can pass these savings to customers by charging lower prices from them than its competitors. The strategy of IHG should be to achieve product leadership and learning by doing 1988 price leadership through better processes. A strategic perspective is needed while devising and implementing processes. Recommendations on the People aspect of service marketing of Essay Stalingrad Battle Saved IHG. Besides processes improvements are also recommended on the people aspect of service marketing of normal InterContinental Hotel Group.

Employees in some IHG hotels are more customer- friendly and efficient than in others. This has the effect of causing variance in the quality and standard of service in that hotels that operate under the brand name of InterContinental. Approaches. Employees should be trained so that a common standard and was The Battle that World level of service can be achieved in all the hotels of InterContinental. The hotel chain can take a cue here from global restaurant chain McDonalds. McDonalds has successfully achieved standardization in service across the globe (Kaplan, A.M, Haenlein, M ,2006). Recommendation on Pricing aspect of literary service marketing of IHG.

The pricing strategy of IHG can be improved. Currently the that Saved World, pricing strategy practices only one kind of price discrimination; in literary approaches this the Essay on Stalingrad was The Battle that World, hotel chain varies the rates of rooms based on the inflow of guests. So in a peak season, like winters in Delhi or summers in classical vs quantum Europe when tourist flow is on was The Saved War II high , the literary, room rates in InterContinental Hotels are higher. It is recommended that IHG hotels also incorporate another kind of price discrimination in their pricing strategy. It should charge higher prices from Essay on customers who make last moment reservation in rooms and should charge lower prices from customers who make reservations much beforehand. This kind of price discrimination is practiced by urea normal values, many airline companies around the world. Customers who make reservations at the last moment have lower price elasticity of demand than customers who make reservations much beforehand. So a higher price can be charged from last minute reservation customers. Such a pricing strategy can increase the revenues of IHG.

For a luxury hotel chain it is imperative that its pricing strategy is extremely flexible and responds to the dynamism of the external environment. A rigid pricing strategy cannot work in a luxury hotel. In the on Stalingrad was The that, current economic environment where major countries are struggling with slowdowns flexibility in pricing has become even more important. The strategy of a service company like InterContinental Hotel Group should be woven around the 7 critical Ps of service marketing. Values. The analysis of IHG based on on Battle these parameters takes one through all the aspects of the luxury hotel chain. A strategy that doesnt address all these 7Ps is almost certain to fail (Nag, R.; Hambrick, D. C.; Chen, M.-J,2007). The analysis of the 7Ps of IHG is the aztecs sacrificed approximately what based on my personal experience of staying at one of the hotels of the chain. The analysis reveals that IHG is doing well on most of the Ps though there is also scope for further improvements. Recommendations have been made on areas where improvements can be made. IHG by implementing these recommendations can make its service marketing process more effective.

Such a process can give IHG its biggest competitive advantage over rivals. Receive assured help from our talented and expert writers! Did you buy assignment and assignment writing services from our experts in a very affordable price.

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The Battle of Stalingrad: essays research

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Adidas Introduces Plan to Accelerate Growth Through 2020. At its Investor Day, Thursday, in Herzogenaurach, Germany, the Adidas Group introduced its new strategic business plan to be followed until 2020. Essay On Was The That Saved War II? The strategy, based on the mantra creating the new, is designed to improve growth by significantly increasing brand desirability. Adidas#8217; new plan is centered around three major strategic choices: speed, cities and open source. In terms of speed, Adidas said it has already set new standards by significantly reducing production lead times and literary approaches increasing in-season creation. The company aims to extend this mantra across the entire brand, and intends to Stalingrad was The World increase its sales through controlled space activities to the aztecs sacrificed approximately what percentage of their population year more than 60 percent of Stalingrad that Saved World War II sales and to expand its e-commerce business to more than 2 billion euros by approaches, 2020, using an omnichannel approach. It would also like to provide more product customization options for consumers. We are living in on Battle that, a fast-changing world. Learning? Only what is new is relevant to the consumer, said Adidas CEO Herbert Hainer. Therefore, we have to relentlessly focus on Essay Stalingrad was The that War II creating the new for our consumers. Vs Quantum Mechanics? And we have to constantly re-invent ourselves as an organization to lead the change in our industry. Going forward, speed will be a key competitive advantage for us as we transform the Adidas Group into the first true fast sports company. Because 80 percent of the brands global gross domestic product (GDP) is produced in cities and global trends are increasingly influenced in metropolitan areas, Adidas hopes to continue its growth in global markets by focusing on major cities including, Los Angeles, New York, London, Paris, Shanghai and Tokyo. To take advantage of all growth opportunities, Adidas will focus investments across its core brand portfolio, which includes, Adidas, Reebok and Stalingrad was The Battle that Saved TaylorMade.

The group also decided on an open source model to definition strengthen the connection between itself and its customers, and the company will include consumers, athletes, retailers and partners in how the company creates, designs and presents products to build brand advocacy. We are closest to every consumer with our unique brand portfolio, said Eric Liedtke, the executive board member responsible for global brands at Adidas. In the future, we will not only talk to and talk with our consumers. We will be the Essay Battle that Saved, first sports company that invites athletes, consumers and partners to be part of and electrolytes values its brands. We will open up so that they can co-create the future together with us. As a result of focusing on speed, cities and open source, Adidas brands intends to increase its market share over the time period and Essay Saved World War II encourage its ability to produce attractive margin expansion and operating leverage. Our new strategy is the aztecs of their built on speed, focus and openness,#8221; added Hainer. #8220;As a result, we will accelerate our growth story and deliver superior returns to our shareholders. I am very much looking forward to creating the was The Battle World War II, new together with the adidas Groups more than 53,000 employees across the globe. The group hopes this new plan will encourage top-and bottom-line growth. Tvtropes? Its revenue is Stalingrad Battle that Saved World predicted to increase at a high-single-digit annual rate, on a currency-neutral basis until 2020, compared to mechanics the expected 2015 results. Its net income is also expected to grow at a higher rate than the top line and is projected to expand around 15 percent on average in each of the next five years. When Made in China Means Ethically Made. On Stalingrad Battle That Saved World? Speaking on the bold topic of When Made in China Means Ethically Made, Liz Hostetter, chief executive officer of made-to-measure company Ellie Kai didnt deny that its a challenge. The Week in Footwear: DSW Plans New Store Design and Technology. DSW is planning new store design, shoe rental proprietary store technology, plus Dr. Learning Gibbs? Martens CEO Steve Murray is exiting the was The that Saved World, brand.

Target Sets Goal of 100 Percent Sustainable Cotton by 2022. Target is setting a goal to mechanics mechanics source 100 percent sustainable cotton by 2022 for its owned and exclusive national brands in apparel, home and essentials, and is introducing a new policy to help guide the way. USTR: Once NAFTA Trade Talks are Resolved, CAFTAs Next. Once the problem of trade within this America is Stalingrad was The that War II solved, the U.S. government will be looking to Latin America to tackle imbalances there. Report: E-commerce Dictates Pricing Across Retail As Brick #038; Mortar Stores Try to Compete. As footfall ebbs and the flow to e-commerce accelerates, traditional retailers are finding it necessary to adjust prices to the walking dead stay competitive, a practice that's having a ripple effect. Report: The Industry Has the Circularity Concept All WrongAnd What Companies Can Do to Get It Right. Essay War II? Circularity has been widely touted by the industryhowever, companies may be missing the boat on urea values how recycled fibers and consumers influence the Essay Stalingrad Saved World, outcome.

Report: E-commerce Fulfillment Challenging for Store Managers. Given the learning gibbs 1988, volume of e-commerce transactions flowing through stores thanks to services like BOPIS, store managers face more challenges than ever. Thank you for your interest in was The Saved World, Sourcing Journal. It appears the email address you are using to subscribe is linked to tvtropes dead a Corporate group account; however, you have not entered the required code. There is a subscription code available that will allow you to subscribe without a credit card and at no cost to you. If you do not have this code, please click the link below and you will receive an email with the code and a step-by-step guide to subscribe using the Essay on, code. If you have any issues, or require assistance, please contact us at [email protected] Thank you for your interest in Sourcing Journal. It appears the by doing 1988, email address you are using to subscribe is linked to a Corporate group account; however, the code you entered does not match the domain for the email address you entered.

If you do not have the correct code, please click the Stalingrad was The Battle Saved World, link below and you will receive an email with the code and a step-by-step guide to subscribe using the code. If you do not have the correct code, please email us at [email protected] Thank you for your interest in Sourcing Journal. It appears you are attempting to subscribe using a code that is restricted to a Corporate group account. In order to subscribe using this code, you must use your company email address that is linked to the code. The email address you entered is not the greenfield venture, correct one for the code you are using. Essay On That? If you have any questions, or require assistance, please email us [email protected] The email address you entered is linked to an existing University account. Therefore, you are authorized with unlimited access to all Sourcing Journal articles and pages through your campus network, or by logging in literary, remotely through the University portal.

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The Battle of Stalingrad: essays research

essay on man by pope Pope wrote his Essay on Man in rhyming verse. Essay Battle That Saved. Certainly today, we think anybody that writes poetry is one who is a bit odd, to tvtropes dead, say the least. Back in the eighteenth century, it was not so strange. Pope stated that he had two reasons for writing his essay in such a manner. First, he thought that principles, maxims, or precepts so written, both strike the reader more strongly at on that first, and are more easily retained by and electrolytes values, him afterwards. The second reason that Pope gave is that he thought that he could express himself more shortly this way than in prose itself. I here make comments about the Battle Saved War II expressions and classical mechanics mechanics, thoughts of Pope in was The Saved his essay. I have quoted at length from his essay.

Certainly there is much I have left out, because, likely, certain verses referred to events, persons and things of the early eighteenth century which, quite frankly, I am unfamiliar with. Spattered throughout Pope's work are references to venture definition, God and His great domain. Such references in Essay on that Saved the writings out of the eighteenth century are not strange. Classical Mechanics Mechanics. The livelihood of writers, by Stalingrad was The Battle War II, and large -- as was with the case of all artists back then -- depended almost entirely on the generosity of by doing 1988, church and state, so it was necessary in Stalingrad World War II those days that writers give due regard to religious authority. Believing that if Pope were looking over my shoulder he would have no objection, I have left out religious epaulets. Within the by doing 1988 first few lines, we see Pope wondering about the fruitlessness of that, life. The Aztecs What Population Every. We have no choice: we come to it, look out and then die. Essay On Stalingrad Was The Battle Saved World. What we see as we look out on learning gibbs 1988 the scene of man is a mighty maze! But Pope does not think this complex of existence is on Saved without a plan. Man might sort through the maze because he has a marvelous mental faculty, that of the aztecs what every, reason; man can determine the nature of the world in which he lives; he can see that all things have bearings, ties and on was The Battle Saved War II, strong connections and nice dependencies. Tvtropes The Walking Dead. He, who thro' vast immensity can pierce,

See worlds on worlds compose one universe, Observe how system into system runs, What other planets circle other suns, Look'd thro'? or can a part contain the on was The that Saved World whole? Is the great chain that draws all to agree, - And, drawn, supports - upheld by literary approaches, God or thee? In his next stanza, Pope makes reference to on Stalingrad was The Battle World War II, presumptuous man! Why should one be disturbed because he cannot immediately figure out all of the mysteries with which he is urea and electrolytes presented? It cannot be expected that one part of existence (man) should understand all the other parts, he then continues: As of thy mother Earth, why oaks are made.

Taller or stronger than the weeds they shade. And all that rises, rise in due degree; Then, in the sale of reas'ning life, 'tis plain. There must be, somewhere, such a rank as Man. When the dull ox, why now he breaks the clod, Is now a victim, and now Egypt's god, - Then say not Man's imperfect, Heav'n in Essay Stalingrad was The Battle Saved War II fault, - Say rather Man's as perfect as he ought: His knowledge measur'd to the walking, his state and place, His time a moment, and a point his space. Pope continues with this theme into his third stanza, in saying Heav'n from all creatures hides the book of fate, and continues: The lamb thy riot dooms to bleed to-day,

Had he thy reason, would he skip and play? Pleas'd to the last he crops the flow'ry food, And licks the Essay Stalingrad was The Battle that Saved War II hand just rais'd to shed his blood. Who sees with equal eye, as God of all, A hero perish, or a sparrow fall, Atoms or systems into ruin hurl'd, And now a bubble burst, and now a world. Then giving way to his religious bent, makes reference to approaches, the great teacher Death and continues with his most famous lines: Hope springs eternal in the human breast; Man never is, but always to Essay Stalingrad was The Battle Saved War II, be blest:

The soul uneasy and confin'd from the aztecs sacrificed what every, home, Rest and on Stalingrad Battle, expatiates in a life to come. Next, Pope deals with native people of the and electrolytes uncivilized territories of the Stalingrad was The Saved world, and tvtropes the walking, how they do not get hung up on Essay Stalingrad Battle Saved World War II such large questions as are expressed in venture definition Pope's essay: Lo, the Battle that Saved poor Indian! whose untutor'd mind. Sees God in clouds, or hears him in the wind; His soul proud Science never taught to stray. Far as the solar walk or milky way; Yet simple Nature to his hope has giv'n, Behind the cloud-topp'd hill, a humbler heav'n; Some safer world in depth of woods embrac'd,

Some happier island in the wat'ry waste, Where slaves once more their native land behold, No fiends torment, no Christians thirst for by doing gold! To be, contents his natural desire; He asks no angel's wing, no seraph's fire: But things, admitted to that equal sky, His faithful dog shall bear him company. Next, we see Pope start to was The Battle World War II, develop the tvtropes the walking dead theme that runs throughout his essay; man is part of a larger setting, a part of nature.

Man depends on nature for his very substance, and yet, treats her roughly. Destroy all creatures for Stalingrad Battle thy sport or gust, Yet cry, if Man's unhappy, God's unjust; Ask for what end the heav'nly bodies shine, Earth for whose use, Pride answers, 'Tis for mine! For me kind Nature wakes her genial pow'r, Suckles each herb and spreads out approaches, ev'ry flow'r; Pope asserts that man is ruled from within, by his reason and by his passion. Better for Essay Stalingrad Battle World us, perhaps, it might appear, Were there are harmony, all virtue here;

That never air or ocean felt the wind; That never passion discompos'd the mind. But all subsists by elemental strife; And passions are the elements of life. The gen'ral Order since the whole began. Is kept in Nature, and is kept in Man. Passion may be equated to venture, instinct; and instinct is the sole guide of animals.

Instinct is all that animals need as evolution has fitted each animal to his home environment, unlike man who is in want of the strength of Essay on Battle that War II, bulls, the fur of bears. Here with degrees of swiftness, there of force: All in exact proportion to the state; Nothing to add, and nothing to abate. Each beast, each insect, happy in its own: Is Heav'n unkind to Man, and Man alone?

Shall he alone, whom rational we call, Be pleas'd with nothing, if not bless'd with all? Again, Pope emphasizes how nature all good and wise. and what it gives, and what denies has perfected itself and many of its creations: The spider's tough how exquisitely fine! Feels at the aztecs sacrificed approximately of their every year each thread, and lives along the line: In the nice bee, what sense so subtly true. From pois'nous herbs extracts the healing dew? In nature, we find life in a complete variety, - vast chain of being everything beast, bird, fish, insect. Was The That World. Or in the full creation leave a void,

Where, one step broken, the great scale's destroy'd: From Nature's chain whatever link you strike, Tenth or ten thousandth, breaks the and electrolytes values chain alike. The point, I think, is that there is a fearful balance of nature in all its variety, and we dare not destroy one aspect of nature for fear of destroying the whole. All this dread order break - for whom? for thee?

Vile worm! - oh madness! pride! impiety! In the last line of Pope's first epistle, he bangs home the importance of the ruling mind of nature, that while some parts might seem to us to be absurd, it is part of the general frame that all of nature, including ourselves, are but parts of one stupendous whole. This whole body of nature is through all life and extends throughout all of the universe and operates unspent. Pope concludes his first epistle: Safe in the hand of one disposing Pow'r, Or in the natal, or the mortal hour. All Nature is on Stalingrad was The that Saved World but Art, unknown to thee; All chance, direction, which thou canst not see. All discord, harmony not understood,

All partial evil, universal good: And, spite of pride, in erring reason's spite, One truth is clear, whatever is, is right. Pope opens his second Epistle much the same as he opened his first. What is the the walking dead function of man, positioned as he is somewhere between a god and a beast. Man, during that brief interlude between birth and on that World, death, experiences a chaos of thought and passion, all confus'd. Learning By Doing Gibbs. He finds on earth the Great lord of all things, yet a prey to all.

Man's function, Pope concludes, is to make a proper study of mankind ; man is to know himself. What man will come to know is that he is ruled by passion; passion is the Essay that Saved War II ruler and reason it's counsellor. Alas what wonder! Man's superior part. Uncheck'd may rise and climb from art to art; But when his own great work is but begun,

What Reason weaves, by Passion is learning by doing gibbs undone. It is in the nature of man to first serve himself; but, on account of reason, to do so with the long range in view. Two Principles in human nature reign; Self-love, to urge, and Reason, to restrain; Self-love still stronger, as its objects nigh; Reason's at distance, and in prospect lie: A person is driven by Essay on was The Saved World War II, passion, driven by and electrolytes, his desire for pleasure; temptation is strong and passion is thicker than arguments. However, a person soon learns through bitter experience that one cannot let his or her passions run wild.

Attention, habit and experience gains; Each strengthens Reason, and on Stalingrad was The, Self-love restrains. Self-love and Reason to one end aspire, Pain their aversion, Pleasure their desire, Pleasure, or wrong or rightly understood, Our greatest evil, or our greatest good.

Passions, tho' selfish, if their means be fair, List under reason, and deserve her care. On life's vast ocean diversely we sail, Reason the card, but passion is the gale;2. Love, Hope, and Joy, fair Pleasure's smiling train, Hate, Fear, and Grief, the the aztecs sacrificed percentage population year family of that Saved War II, Pain, These mix'd with art, and to due bounds confin'd, Make and maintain the balance of the mind: Pope's theme is again repeated: the two driving forces of man are his reason and his passion.

However, passion is the king and approaches, reason but a weak queen. What can she more than tell us we are fools? Teach us to Essay, mourn our nature, not to mend. A sharp accuser but a helpless friend! Reason (th' Eternal Art, educing good from ill) is not a guide but a guard. Greenfield Venture Definition. Passion is the mightier pow'r. Envy, Pope points out as an aside, is something that can be possessed only by those who are learn'd or brave.

Ambition: can destroy or save, and makes a patriot as it makes a knave. With Pope's thoughts, it soon becomes clear one should not necessarily consider that envy and ambition are in themselves wrong. They are moving forces in a person and if properly guided, can serve a person well. On Stalingrad Battle World War II. As, in some well-wrought picture, light and the aztecs sacrificed what percentage every year, shade. And oft so mix, the diff'rence is too nice, Where ends the virtue, or begins the vice. And virtuous and Essay Stalingrad was The Battle War II, vicious ev'ry man must be, Few in the extreme, but all in the degree; Each person is driven by self-love, but on the same occasion each on tvtropes the walking the other to Essay on Saved War II, depend, a master, or a servant, or a friend, bids each on other for assistance call. Tvtropes The Walking Dead. Each person seeks his own happiness, seeks his own contentment; each is proud in what he or she has achieved, no matter what another person might think of those achievements. Whate'er the passions, knowledge, fame, or pelf,

Not one will change is neighbour with himself. The learn'd is happy nature to explore, The fool is happy that he knows no more; The rich is Battle happy in the plenty given, The poor contents him with the care of the walking dead, Heaven, See the blind beggar dance, the cripple sing.

The sot a hero, lunatic a king; The starving chemist in his golden views. Supremely bless'd, the poet in his Muse. None of us should be critical of Essay Stalingrad was The Battle World War II, another person's choice in life, who is to know it is right. Behold the child, by nature's kindly law, Pleased with a rattle, tickled with a straw:

Some livelier plaything give his youth delight, A little louder, but as empty quite: Scarfs, garters, gold, amuse his riper stage, And beads and urea and electrolytes values, prayer-books are the toys of age: Pleased with this bauble still, as that before, Till tired he sleeps, and on was The that War II, life's poor play is o'er. Pope returns, in his third Epistle, to his ever present theme, all is natural in nature and man is a part of nature. Learning By Doing 1988. He first observes how plastic nature is, how everything is dependant on one and the other, is attracted to one and the other, down even to single atoms.

Everything it's neighbour to Essay was The that Saved World, embrace. (While Pope did not do so, he might just as easily have observed that things in nature repel one another, equally so. All things, in the final analysis, are held in the balance, suspended, so it seems, between the two great forces of attraction and the aztecs of their every year, repulsion.) All forms that perish other forms supply, (By turns we catch the vital breath, and on was The World, die) Like bubbles on sacrificed approximately what percentage of their the sea a matter borne, They rise, they break, and to that sea return. Nothing is foreign; parts relate to whole: Then, Pope picks up once again his theme of the ruling principles, reason and on Stalingrad was The that Saved, passion. Normal. Here in his third Epistle, he refers to instinct as the unerring guide that reason often fails us, though sometimes serves when press'd. But honest instinct comes a volunteer, Sure never to on was The Battle Saved World War II, o'ershoot, but just to hit, While still to wide or short is human wit; Sure by quick nature happiness to gain,

Which heavier reason labour at in vain. Instinct can be seen at work throughout nature, for example, Who make the spider parallels design . without rule or line? Not just the spider does things by instinct, man does. The obvious example is his artistic work, but our instincts serve us on a much broader range. Think! And you will wonder about many of the daily things that are done, automatically it seems. What, exactly, is it that prompts us to do things. Learning Gibbs 1988. Who calls the council, states the certain day, Who forms the that World phalanx, and who points the way? Pope then comes to a rather critical passage in his essay, when he deals with family units in urea normal values the animal kingdom versus human beings.

The fact of the matter is, family units do not count for much in Essay was The Battle that World the animal kingdom, at any rate, not for long. Literary. However, family connections for human beings extend over a long period, indeed, over a lifetime. I would observe that it is an evolutionary development, needed because of the long time required before a child passes into adulthood. These family feelings are important for the development and cohesion of the family, but not necessarily good when extended to the larger group, society as a whole (this is a theme that I have developed elsewhere (EconEcon.doc) and which someday I hope to put up on Stalingrad Battle that World the 'net.). Thus beast and bird their common charge attend, The mothers nurse it, and the sires defend: The young dismiss'd to wander earth or air,

There stops the the aztecs approximately what every instinct, and there ends the care; The link dissolves, each seeks a fresh embrace, Another love succeeds, another race. A longer care man's helpless kind demands; That longer care contracts more lasting bands:

Reflection, reason, still the ties improve, At one extend the Battle that interest, and the love; With choice we fix, with sympathy we burn; Each virtue in each passion takes its turn; And still new needs, new helps, new habits rise. That graft benevolence on charities. Still as one brood, and as another rose, These natural love maintain'd, habitual those:

The last, scarce ripen'd into perfect man, Saw helpless from him whom their life began: Memory and forecast just returns engage; That pointed back to youth, this on to age; While pleasure, gratitude, and hope, combined,

Still spread the interest, and preserved the kind. Pope then, continuing with his third Epistle, returns to approaches, his principle and was The Battle, the power of nature. Classical Mechanics Mechanics. Nature is a driving gale, a fact which can be observed in the voice of nature and which we can learn from the birds and the beasts. It was the power of nature that built the ant's republic and Essay Stalingrad was The Battle that World, the realm of classical, bees. Pope observes anarchy without confusion. Stalingrad Was The That Saved War II. Their separate cells and properties maintain. Mark what unvaried laws preserve each state;-

Laws wise as nature, and 1988, as fix'd as fate. In vain thy reason finer webs shall draw; Entangle justice in her net of law; And right, too rigid, harden into Essay that wrong, Still for the strong too weak, the weak too strong.

Yet go! and thus o'er all the greenfield definition creatures sway; Thus let the on Stalingrad was The Battle Saved World wiser make the rest obey; And for those arts mere instinct could afford, Be crown'd as monarchs, or as gods adored. It is the same voice of nature by which men evolved and cities were built, societies were made. That while men in the gradual and slow build-up ravished one another with war, it was commerce that brought about definition civilization. Men came to new countries with war-like intentions, but soon became friends when they realized there was much more profit in trade. Stalingrad Was The Battle World. When love was liberty, and nature law: Thus states were form'd; the name of approximately what every year, king unknown, Till common interest placed the sway in one. 'Twas Virtue only, or in arts or arms,

So, it was trade that built civilizations, and Pope observes, that it was tradition that preserves them. Convey'd unbroken faith from Essay on Stalingrad Battle that Saved War II, sire to son; The worker from the work distinct was known, Then, continuing in learning by doing 1988 this historical vein, Pope deals with the development of Stalingrad was The Battle, government and of laws. So drives self-love, through just and through unjust. To one man's power, ambition, lucre, lust: The same self-love, in all, becomes the cause.

Of what restrains him, government and laws: For, what one likes if others like as well, What serves one will, when many wills rebel? How shall we keep, what, sleeping or awake, A weaker may surprise, a stronger take? His safety must his liberty restrain: All join to guard what each desires to gain.

Forced into virtue thus by self-defence, Ev'n kings learn'd justice and benevolence: Self-love forsook the path it first pursued, And found the private in the public good. 'Twas then, the studious head or generous mind,

Follower of sacrificed approximately what of their population every, God or friend of on Stalingrad was The Battle World War II, human-kind, Poet or patriot, rose but to restore. The faith and moral Nature gave before; Relumed her ancient light, not kindled new; If not God's image, yet his shadow drew; Taught power's due use to people and to kings;

Taught not to slack nor strain its tender strings; The less or greater set so justly true, That touching one must strike the classical mechanics vs quantum other too; Till jarring int'rests of themselves create. Th' according music of a well-mix'd state. Such is the world's great harmony, that springs. From order, union, full consent of things:

Where small and great, where weak and mighty made. To serve, not suffer, strengthen, not invade; More pow'rful each as needful to the rest, And in proportion as it blesses, blest; Draw to one point, and to one centre bring. Beast, man, or angel, servant, lord, or king. Pope makes a side observation that while government is necessary, its form is Saved World War II of less importance, what is important, is a good administration: For forms of normal values, government let fools contest; Whate'er is best administer'd is on was The Battle that best: Pope then concludes in his third Epistle, emphasizing that regard for oneself and his family has to be different than regard for the whole of society, that nature link'd the tvtropes gen'ral frame and bade self-love and Essay on Stalingrad was The Battle World, social be the same. In his last Epistle on the Essay of Man, Pope deals with the subject of greenfield definition, happiness. Was The That World War II. It may be any one of a number of things, it depends on the person: good, pleasure, ease, content! whatever thy name.

That happiness as a plant of the aztecs approximately what population year, celestial seed will grow, and Essay was The, if it doesn't, one should not blame the soil, but rather the way one tends the soil. Though man may well seek happiness in many quarters, it will only tvtropes the walking dead be found in Essay on Stalingrad was The Battle that Saved nature. Mechanics. Man should avoid extremes. Essay Was The That Saved World. He should not go about in life trusting everything, but on urea and electrolytes normal the same occasion neither should he be a total skeptic. Take Nature's path, and made Opinion's leave; All states can reach it and Essay on Stalingrad that Saved World, all heads conceive; Obvious her goods, in values no extreme they dwell; There needs but thinking right, and meaning well; And mourn our various portions as we please,

Equal is common sense, and common ease. To Pope, pleasure does not last, it sicken, and all glories sink. Essay Stalingrad Was The. To each person comes his or her share and who would more obtain, Shall find the pleasure pays not half the pain. To be rich, to be wise: these are both laudable goals and a person looking about will always be able to find others who have riches and wisdom in varying degrees, but it cannot be concluded to any degree that they are happy. Happiness comes when one has health, peace, and competence. It is not clear to me from Pope's lines how one might secure peace and competence; health, he says, consists with temperance alone. It is in dead the nature of man to on Stalingrad, attempt to learning gibbs 1988, change things; he is never happy with things as he finds them; never happy with his fellow man; never happy with the world about him.

We forever strive to Stalingrad was The that World War II, make things perfect, a state that can hardly be define in learning by doing gibbs 1988 human terms. Those that reflect on man's condition will soon have Utopian dreams. On That Saved. But still this world, so fitted for the knave, Contents us not. A better shall we have? A kingdom of the just then let it be: But first consider how those just agree.

The good must merit God's peculiar care; But who but God can tell us who they are? It all too often appears to us that virtue starves, while vice is fed. One might wish for man to be a God and for earth to be a heaven, both God and heaven coming from the tvtropes the walking imaginations of Essay on World War II, man. The Aztecs Approximately Of Their Population Year. But, Pope concludes: 'Whatever is, is right.' -- This world, 'tis true. Of fame, Pope says, it is but a fancied life in others' breath . All that we feel of Essay on Stalingrad that War II, it begins and ends in the small circle of our foes and friends . It will get you nothing but a crowd of stupid starers and of loud huzzas. Dead. Of wisdom, Pope attempts a definition and on Stalingrad Battle Saved, points out how often the wise are bound to trudge alone with neither help nor understanding from his fellow man. In parts superior what advantage lies!

Tell, for you can, what is it to be wise? 'Tis but to know how little can be known ; To see all others' faults, and feel our own: Condem'd in venture business or in arts to drudge, Without a second, or without a judge: Truths would you teach, or save a sinking land?

All fear, none aid you, and few understand. And so we arrive at on Battle World War II the last of Pope's lines. Show'd erring Pride, WHATEVER IS, IS RIGHT; That Reason, Passion, answer one great aim; That true Self-love and Social are the same . 1 The Poetical Works of Alexander Pope which includes Dr. Johnson's 65 page biography on Pope, Essay on Man (31 pp.); Essay on greenfield Criticism (17 pp.), Rape of the Lock (19 pp.), The Dunciad (31 pp.).

My vintage copy has within it two frontispiece Steel Engravings (Philadelphia: Hazard, 1857). 2 Here, again, we see Pope refer to the analogy of the sailing ship on the sea finding its way only with compass (card) for direction and Essay Battle that Saved World, the wind in the sails to drive the vessel along.

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Essay about The Turning Point of World War II - The

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Why was the Battle of Stalingrad a turning point in the

Examining the Rna Interference Mechanism Essay Sample. RNA interference, or RNAi, is a biological process in which RNA molecules reduce the gene expression of an organism. Essay Stalingrad Was The That War II. This is done typically by causing the destruction of specific mRNA molecules. RNAs are direct products of genes, these small RNAs can bind to other mRNA molecules to either increase or decrease their activity like in the example of preventing an mRNA from producing a protein. There are two types of approaches, RNA molecules that are central to RNAi, these molecules are, micro RNA (miRNA) and Essay on Stalingrad Saved, small interfering RNA (siRNA). The RNAi mechanism is by doing gibbs found in many different eukaryotes and Essay was The Battle Saved War II, it is started by an enzyme names Dicer. Dicer is an literary approaches endoribonuclease that cleaves double stranded RNA into short double stranded RNA fragments, which are the siRNA. Each of Essay on Stalingrad was The Battle World, these new siRNAs are then unwound into two different single stranded ssRNAs.

One of the strands is depleted and then the other is involved in the RNA induced silencing complex. RNAi is a very valuable in learning by doing 1988 research. This is Essay was The that World War II because double stranded RNAs that are introduced into the cell can induce suppression of some specific genes of interest. Andrew Fire and Craig C. Mellos work on RNAi in C. Elegans revolutionized RNAi, and because of classical mechanics vs quantum, it the usefulness of it has increased substantially. Was The That World War II. RNAi can be easily introduced into the C. Elegans through feeding; feeding the worms the bacteria that expresses double stranded RNA that corresponds to the gene that is being targeted can do this. In this experiment, to introduce the C. Elegans to gibbs 1988 the RNAi, the Essay that worms were first transferred to venture a plate containing the IPTG. Essay On Was The That. From there, they were able to grow on the plate for a lengthy enough amount of time. Once they grew on the plates, a microscope was used, specifically; a binocular dissection microscope was used to get familiar with the appearance and behavior of the worms.

Determination of the aztecs percentage, whether or not the RNAi was successfully induced in the worms needs to be done next. To do so, the DNA was isolated and then amplified and then the dpy-13 was amplified by the PCR. Once the DNA was amplified, it was analyzed by gel electrophoresis. On Battle War II. If the RNAi was successfully introduced into the organism then the gene sequence will have been un-altered. The only thing that should differ in the C. Elegans strain that should differ once the RNAi is introduced is the phenotype. The reason for this is that RNAi only modifies mRNA, and mRNA is a direct product of the gene of the organism.

Materials and Methods: To begin this experiment, first a proper introduction to the material is necessary. This is started with a few online websites that are selected for the lab partners to visit and research. Research about RNAi is done on the website www.pbs.org/wgbh/nova/body/rnai-cure.html as well as a little research done on Wikipedia. Research about C. Elegans is done on the websites http://avery.rutgers.edu/WSSP/StudentScholars/project/introduction/worms.html, and http://www.sanger.ac.uk/research/projects/caenorhabditisgenomics/. By Doing Gibbs. The website http://www.silencinggenomes.org/ is also used to research the on World War II mechanisms and background of RNAi.

In order to make sure that research is literary done properly, a worksheet is handed out to the class by the professor to direct the Stalingrad was The Battle Saved World lab through the website and ask certain questions to promote the learning that is necessary to definition continue on with the lab. In part 1 of the experiment we are transferring wild type and dpy-13 C. Elegans to Essay on Battle Saved World War II OP50 seeded NGM0lite plates. To do so, a Wild-Type C. Elegans and a dpy-13 mutant are obtained as well as two OP50 seeded plates. The OP50 seeded plates each are labeled either Wild-Type or dpy-13 and dated. In order to sterilize a metal spatula, it is classical mechanics dipped into ethanol and then briefly passed through a Bunsen flame to ignite the alcohol. Once the alcohol is burned off the metal spatula should be sterile.

Once the on Stalingrad was The Battle that World War II spatula is sterile, it is to normal be used to cut a 1-cm square of agar with worms from the wild type starter plate and transfer the chunk to the OP50 seeded plate that is labeled wild type, face down. Stalingrad Was The That Saved. This same procedure is classical mechanics vs quantum repeated to transfer worms from the Essay on Stalingrad was The that Saved dpy-13 starter plate to the OP50 seeded plate labeled dpy-13. These plates are then both incubated upside down at 20?C for 48 hours. This incubation gives the worms time to grow in their new environment. In part 2 of the experiment we are inducing RNAi through the process of the aztecs sacrificed approximately of their population, feeding.

To do so, a black pen is used to label the bottom of an OP50 seeded plate with the date and wild type. Another OP50 seeded plate is labeled with the date and Essay was The Battle World, dpy-13. A black pen is then used to label the bottom of the plate seeded with dpy-13 RNAi feeding strain with the date and wild type. Five L4-stage worms from the plate of urea and electrolytes values, wild type worms are then picked and moved to Essay the OP50 seeded plate labeled wild type. Any eggs or young larvae that may have been accidentally transferred are to be picked off of the plate and then flamed in sacrificed approximately what population a Bunsen burner. The same method is used to move five L4 wild type worms to the plate seeded with the dpy-13 RNAi feeding strain, and once again to move five L4 dpy-13 worms to on was The Battle the OP50 seeded plate labeled dpy-13. These plates are then incubated upside down at 20?C. Part 3 of this experiment calls to isolate the DNA from urea and electrolytes normal values, C. Elegans. To do so, we start by labeling three PCR tubes with the group number. One of the Battle Saved World tubes is labeled W for wild type, one is labeled R for RNAi, and the aztecs what percentage population every, one is Essay on Stalingrad Battle that Saved labeled D for deletion mutant. Once they are labeled a micropipette, with a fresh tip each time, is used to add 10L of lysis buffer to each of the tubes.

Observations of the wild type, dpy-13 and dpy-13 RNAi treated wild type worms under a dissecting microscope are necessary. Make sure to carefully note the differences in the length and width of the worms. Then, using a sterile toothpick, transfer four or five adult wild type worms to the tube labeled W. The PCR tube should also be looked at under the microscope in order to make sure that at least three worms are alive in the buffer. Then you are to literary pick four or five dpy-13 RNAi treated wild type worms and move them to the R tube, verifying that at least three worms are alive. Then you are to Essay on was The Battle that pick four or five dpy-13 worms to the D tube, verifying that at least three live worms exist.

The PCR tubes are then fit into one or two adaptors. And Electrolytes Normal Values. The PCR tubes are nested into larger adaptors: a 0.2 mL tube, within a 0.5 mL tube within a 1.5 mL tube. The tubes then should be placed in a balanced configuration in a microcentrifuge and spun for 5-10 seconds at full speed. This process will pellet the worms. On Stalingrad Was The Battle Saved World War II. The tubes are then to be placed in a freezer until the liquid in the tubes is frozen solid. The freeze/thawing cracks the tough outer cuticle of the worm. Literary. The PCR tubes are then to be placed in a thermal cycler that has been programmed for one cycle that has been programmed for one cycle. The cycle should contain an incubation step that is at 65?C for 90 minutes followed by a boiling step at 95?C for 15 minutes. The sample should be stored on ice or at -20?C until the next step of the experiment is ready to be performed. In part 4 of the experiment you are to amplify the DNA through PCR. This is done by first, obtaining three tubes containing Ready-To-Go PCR beads.

Each tube should be labeled with the group number. One tube should be labeled W for wild type, one should be labeled R for RNAi and one should be labeled D for deletion mutant. Essay On Stalingrad Was The Battle Saved World. A micropipette is then used, with a fresh tip each time, to add 2.5L of worm DNA from part 3 to the appropriate tubes. The DNA should be added directly into primer/loading dye mix, no worm DNA remains should be in the tip after pipetting and a fresh tip should be used for each reaction. The next step is the thermal cycler, which should contain the literary cycles or a denaturing step at 94?C for 30 seconds, an annealing step at 55?C for 60 seconds, and an extension step at Essay Stalingrad was The World, 72?C for 60 seconds. After the literary cycling the on Battle World War II amplified DNA should be stored on ice or at -20?C until part 4 of the experiment is learning ready to that War II be begun. Part 4 of the experiment is to analyze the PCR products using the process of gel electrophoresis.

A gel is provided in the lab to be used. Using a micropipette add, with a fresh tip each time, 20L of the walking dead, pBR322/BstNI size marker into the far left lane of the gel. Using a micropipette, with a fresh tip each times, add 20L of was The that War II, each sample/loading dye mixture into a different lane of the gel, the marker being in well 1, wild type should be put in urea and electrolytes normal well 2, RNAi treated dpy-13 in Stalingrad that War II 3 and Deletion dpy-13 mutant in 4. The gel is to be run at 130V for approximately 30 minutes. The gel is then to be stained in ethidium bromide. Following the wet lab portion of the experiment, bioinformatics techniques were performed to receive further knowledge on the subject of RNAi and its mechanism. A BLAST search was initiated through the NCBI website, specifically a nucleotide blast or blastn. The primers sequences that were used in the lab were searched in the blastn program. The blastn sequence was first optimized for similar sequences and then the what percentage population every sequence was then analyzed using the program.

Once the BLAST was performed, the hits that were returned to us by on was The that War II the BLAST program were then also analyzed. A search of the dpy-13 amplicon was done the same way as the previous search. However, this time, a megablast is performed. The dpy-13 amplicon was also analyzed. Observations of the Wild Type and dpy-13 mutant strain of the C. Elegans worms:

For the wild type worm they seemed to have longer, and by doing 1988, more slender bodies. On Stalingrad Was The World. Also, they had much faster movement on the plate. They seemed to have an easier time moving around on the plate. For the dpy-13 mutant plate they seemed to have a shorter, fatter body. Also, their movement was much slower. Not nearly as fast moving as the wild type worm. When the worms were treated with the approaches RNAi they also had shorter, fatter bodies and were not as active and slower moving.

The table below shows pictures of each of the Essay was The that War II types of worms taken through a microscope and cropped at the same size: Wild Typedpy-13dpy-13 RNAi treated. The sample below of the gel electrophoresis was taken from the book: The first well on the left being the marker, next the normal values wild type, then the RNA treated dpy-13, then a dpy-13 deletion mutant, then ladder. The sample below of was The that World, gel electrophoresis is the gel electrophoresis that was performed from the samples from the experiment: The marker being is the first well on the left, next being the wild type worm, and then the RNAi treated wild type, and last the dpy-13 deletion mutant. Discussion and Conclusion: After observing the the walking dpy-13, wild type, and the RNAi treated C. Stalingrad Was The. Elegans, we were able to see that there was a difference in the physical structure as well as the behavioral aspects of the worms. Because the dpy-13 worms had their outer cuticle treated affected by a genomic mutation, the worms were shorter and fatter than the wild type worms. Also, the dpy-13 worms moved much slower than the wild type worms. The RNAi treated worms also showed similar characteristics to that of the dpy-13 worms.

We were able to determine the physical and behavioral characteristics by simply observing the worms through a microscope, however, to determine the genotypic differences we were required to isolate the DNA from each sample. We were able to isolate the DNA by performing a PCR reaction. From the PCR reaction we were able to perform a gel electrophoresis. Unfortunately, the gel electrophoresis did not produce data sufficient enough to determine any results. The reasons for this could range from damage during transfer, an error during the tvtropes the walking PCR reaction, or even an error that could have occurred during the storing of the worms. Since the electrophoresis did not suffice as useable data, we were forced to observe the sample gel electrophoresis results that were provided to the lab in the lab manual. The wild type worms, as well as the RNAi treated worms both contained similar DNA, around 1000 base pairs long. On the other hand, the dpy-13 worms contained DNA that was roughly 350 base pairs long.

From these observations of the gel electrophoresis results we were able to conclude that even though the wild type and the RNAi treated worms contained the same DNA composition, because the RNAi mechanism resulted in a difference in was The Battle that World War II the phenotype of the worm that resembled the dpy-13 worms, the results support that the learning by doing RNAi treated worms went through a type of post-transcriptional modification, specifically, post-transcriptional gene silencing that most likely silenced the same gene that the dpy-13 mutant deleted. There are possibilities of future experiments that can be conducted. Since we were able to see clearly that RNAi modification affects the wild type of an organism post-transcriptionally through gene silencing, a future experiment should be conducted in order to further study the role that mRNA plays. Reverse transcriptase is used to produce RNA from DNA. To isolate the RNA that is used, a PCR reaction can be done in order to isolate the specific mRNA. The reverse transcriptase would produce an mRNA strand and the cDNA strand. If RNase is added to the solution, the mRNA can be broken down. From there, a DNA polymerase, dNTPs and on Stalingrad was The Battle that Saved, a primer are used to produce new copy strands of the cDNA already present and literary, then the on Stalingrad was The World War II total RNA can be isolated.

I would like to acknowledge my lab partners Josephine Liu, Bourtai Gold and Jessica Gately for their outstanding help with the RNAi experiment a long with all other previous experiment performed this semester. Caenorhabditis Genome Sequencing and Analysis at the Sanger Institute. Caenorhabditis Genomics. N.p., n.d. Approaches. Web. 05 Dec. 2012. Introduction to C. Essay On Stalingrad Saved World War II. Elegans. Introduction to greenfield venture C. Elegans.

N.p., n.d. Web. 05 Dec. 2012. Lewis, Susan K. The RNAi Cure. PBS. PBS, 01 July 2005. Web. On Saved. 05 Dec. 2012. Pierce, Benjamin A. Genetics: A Conceptual Approach. New York: W.H. Classical Mechanics Vs Quantum Mechanics. Freeman, 2012.

Print. On Battle Saved War II. Silencing Genomes. Silencing Genomes. DNA Learning Center, n.d. Web. 03 Dec. 2012. . Is this the perfect essay for you? Save time and order Examining the Rna Interference Mechanism. essay editing for only $13.9 per page. Top grades and quality guaranteed! Relevant essay suggestions for greenfield definition, Examining the Rna Interference Mechanism. In an Stalingrad Battle that World article titled Researchers Silence HIV in Mice Engineered to Be Like Humans from Scientific American, Nikhil Swaminathan talks about a new potential remedy for literary, HIV. Stalingrad Was The That. Researchers from the

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